Influencia de la concentración de infusiones de hojas de Quercus sideroxyla en la extracción de compuestos fenólicos, actividad antioxidante y antiinflamatoria
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https://doi.org/10.59741/eb5fkj20Palabras clave:
Infusiones de encino, polifenoles, antioxidante, antiinflamatorioResumen
Las infusiones del encino Quercus sideroxyla por su contenido rico en compuestos polifenólicos han mostrado tener efectos bioterapéuticos importantes, especialmente antioxidantes y antiinflamatorios. Dentro de las variables principales para la extracción de compuestos bioactivos por medio del infusionado en agua resalta la relación soluto-solvente. El propósito del presente trabajo fue analizar la influencia del porcentaje de hojas sobre el contenido fenólico extraído y su potencial efecto antioxidante y antiinflamatorios. Para ello se obtuvieron infusiones de Quercus 1 al 10% (p/v) de hojas, las cuales se analizaron el contenido de fenoles y flavonoides totales, se realizó un seguimiento del perfilado fenólico para ácidos fenólicos, flavonoides y taninos hidrolizables por medio de UPLC-ESI--MS/MS. Se determinó la actividad antioxidante por medio de los métodos de FRAP, ABTS, ORAC y DPPH, así como de la actividad antiinflamatoria por medio de un ensayo de estabilización de membranas de eritrocitos. La variación en el porcentaje de hojas para la elaboración de las infusiones promovió un aumento progresivo de la extracción de fenoles hasta un porcentaje del 7% (p/v), posteriormente no se observaron diferencias significativas, pero si una disminución nominal del contenido. El perfilado fenólico se vio también afectado, favoreciendo una mayor concentración de hojas de encino la extracción de taninos hidrolizables, especialmente vascalagina y penducalagina 1 y 2, los cuales contribuyeron en aumentar la actividad antioxidante para el atrapamiento de radicales peróxido y reducción del ion férrico. La actividad antiinflamatoria también se vio aumentada, aunque es posible que ésta se relacione con un efecto sinérgico entre la epicatequina galato con otros polifenoles.
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Derechos de autor 2025 Carlos Alonso Salas Ramírez, Martha Rocío Moreno-Jiménez, Nuria Elizabeth Rocha-Guzmán, Rubén Francisco González-Laredo, José Alberto Gallegos-Infante, Manuel Efraín González-Mercado, Karen Marlenne Herrera-Rocha
Esta obra está bajo una licencia internacional Creative Commons Atribución-CompartirIgual 4.0.
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